
Hazards
Corrosive Chemicals
Reactive Chemicals
Insidious Hazards
Toxic Hazards
Biological Hazards
Radiation Hazards
A toxic substance has the potential to injure by direct chemical action with body systems. Almost any substance is toxic when taken in excess of tolerable limits. Toxic substances include corrosive as well as poisonous materials.
Toxic materials can enter the body by
The effects of corrosive materials are usually rapid, but the effects of poison may not be immediately noticed. Many substances' effects (e.g., arsenic and mercury) are cumulative and poisoning can be the result of several exposures over a period of time.
Poisoning may be suspected when any of the following are evident. They are
Toxic materials damage the body by interfering with the function of cells in body tissue. Damage can occur when
Toxic effects can be local or systemic as well as acute or chronic. Local effects are confined to the area of the body that has come in contact with toxic materials. Systemic effects occur throughout the body after absorption into the bloodstream. Acute effects are immediate, while chronic effects may take many years before they become evident.
Toxic materials or "controlled products" are rated in Manitoba by an Occupational Exposure Limit (OEL) as defined in sections 19 and 20 of the Workplace Health Hazard Regulation (53/88), a regulation under the Workplace Safety and Health Act (chapter W210).
The OEL is defined as the limit of exposure of a worker in a workplace to an airborne controlled product or an airborne ingredient in a controlled product.
The potential for contact with toxic materials exists in activities suggested within various science curricula. Chemistry experiments are the most obvious situations with potential hazard. However, a person may be exposed to toxic substances from unsuspected sources. Toxic materials may be involved incidentally as part of a laboratory or demonstration procedure. Careful consideration must be given to all materials used and produced in an activity (e.g., the dust of heavy metal minerals may be inhaled during the breaking of rock samples).
Inadequate clean-up may lead to exposure to toxic materials after a lab procedure is finished. Substances left on benches or in beakers and bottles may expose others to these toxic materials. Students may ingest toxic materials they have been in contact with if they do not wash thoroughly before eating or smoking. Foods and beverages readily absorb many vapours and must never be brought into a lab. Chewing of gum should not be allowed.
Teaching About Toxic Hazards
Teachers should draw students' attention to procedures regarding toxic hazards at every opportunity.
Toxic Materials Protection Policy
Accident prevention depends on forethought, identification of hazards, and careful instruction. The onus is on the teacher to be aware of potential dangers and convey this information to students. The teacher must instruct students in proper handling procedures and must insist that they be followed.
The acquisition, use, and storage of toxic materials must be related to real needs. If safe alternatives exist, they should be used. Only minimum quantities should be stored. Stock bottles should not be allowed in the laboratory. Toxic materials should not be used unless there is adequate protection from exposure.
Suggested Lesson Plan:
Consider the time required and allow for frequent reinforcements of safety messages.
Placement:
Laboratory Safety Orientation (every science program)
Senior 1 Science Chemical Interactions
Senior 2 Science Laboratory Safety
The use of protective gear, prescribed handling procedures, and attention to absolute cleanliness must be the normal routine of any laboratory. Maintaining the routines requires safety attitudes that are nurtured by example and constant practice.
Students must be made aware of and obtain knowledge of the hazard presented by any substance they use. Appearance and odour will not always provide signals that indicate poisonous or corrosive substances are present. Most students are surprised by the wide range of dangers that exist when chemicals are incorrectly handled (see Student Handout: Toxic Materials below).
Student Handout: Toxic Materials
Hazardous situations include
Special Caution: Odours and appearance are not reliable guides to the toxicity of substances. What looks like water could be a dangerous acid or base -- or worse. Many toxic vapours have little or no odour, even in dangerous concentrations.
Protection
A variety of measures will provide protection. They include
Accident Procedures
In the event of an accident, students will
Micro-organisms, like toxic chemicals, are a potential hazard to persons performing biological experiments. Working with them requires special handling, storage, and disposal techniques. Teachers must be aware of the hazards presented by infectious agents and their possible sources.
Occurrences of Accidental Infection
In approximately 80 per cent of all laboratory-acquired infections, the cause is unknown. In the remaining 20 per cent of the cases that have known causes, five are most frequent. They are
Other common causes include
Precautions
Specific Laboratory Operations
A number of specific laboratory operations deserve special attention when micro-organisms are involved.
Pipetting--the greatest hazards are
The last two hazards can be eliminated by the use of a pipetting bulb.
To prevent the first hazard (production of aerosols)
Immediately after use, contaminated pipettes should be immersed in a germicidal solution, and then autoclaved.
Syringes--the greatest hazards are
Inoculating Loops
Use care, as the film held by a loop may break and cause atmospheric contamination. A hot loop may cause a liquid to spatter when it is inserted. Allow it to cool first. A contaminated loop may produce an aerosol by boiling and volatilization when it is placed into a flame for sterilization, even before all pathogenic organisms are killed. Whenever inoculating loops are used, any actions that might result in the generation of an aerosol -- jerky motions, shaking the loop, and agitating liquids -- must be avoided.
Teachers should dip inoculating loops into ethanol before flaming (prevents aerosol formation).
Caution: Care must be taken because of the flammability of ethanol.
Centrifuges
Centrifuges can be cleaned with ethanol to kill any bacteria present (use the fume hood).
Growing Bacterial Cultures
There is always the possibility of a few spores of pathogenic bacteria being introduced from the atmosphere. Be sure the culture medium is properly sterilized by autoclaving. After inoculating the medium with bacteria, be sure to wash hands and clean up any spills with a good disinfectant.
It is recommended that disposable petri dishes be used. When finished with the bacterial cultures, the dishes should be collected in a bio-hazard plastic bag and then autoclaved before disposal.
Use of Human Tissue and Fluid
A recent medical review about the potential risk of transmitting hepatitis or AIDS (acquired immune deficiency syndrome) through the extraction and analysis of samples of human fluid or tissue has led to the complete elimination in Manitoba schools of these experiments or demonstrations. Prepared slides for teaching purposes are available from supply houses.
Infectious Agents
Nearly all groups of micro-organisms have some effect on people. The various groups and some of the diseases for which each group is responsible are shown below.
| Micro-organism | Human Disease |
|---|---|
| bacteria | diphtheria tuberculosis rheumatic fever pneumonia |
| viruses | chicken pox measles mumps poliomyelitis |
| fungi | athlete's foot systemic mycosis |
| rickettsiae | typhus Q fever rocky mountain spotted fever |
| protozoa | schistosomiasis malaria giardiasis |
Each group is responsible for many more diseases than listed. All groups of micro-organisms contain some pathogenic members. Consequently, experiments which may involve micro-organisms either directly or indirectly must be strictly controlled.
Teaching About Biological Hazards
Teachers should discuss biological hazards as they appear in the context of laboratory activities.
Suggested Lesson Plan
Placement:
Science 7 Microbes and Ecosystems
Senior 1 Science Safety Orientation
Senior 2 Science Introduction to Biology
Senior 3 and 4 Biology Microbiology Unit
Possible student assignments include
The term radiation can be and is applied to any physical property which "radiates" outward from a source. It is applied to many physical phenomena including the entire electromagnetic spectrum, high energy particulate emissions from radioactive sources, and even sound and ultrasound. Radiation may be transmitted, absorbed, or reflected by a particular medium.
It is generally classified into two distinct groups, Ionizing and Non-Ionizing. The distinction lies in whether or not a particular type of radiation has the ability to strip electrons from the atoms (to "ionize") in a medium in which the radiation is being absorbed. Because of the unique properties of these two classes of radiation, they are discussed separately.
Biological Effects
The process of ionization can break molecular bonds, thereby altering the chemical structure of the target material. If the affected molecule is part of a critical structure within a cell in the human body, the function of that cell may be altered. This may result in cell mutation, cell malfunction, or cell death. Such interactions occur constantly as a result of exposure to naturally occurring background levels of ionizing radiation (e.g., from cosmic rays) and chemical agents. The body has evolved repair mechanisms to correct this damage.
Such cellular alterations may ultimately result in carcinogenesis. No threshold level of radiation exposure exists above which carcinogenesis occurs. Rather, the risk of cancer later in life increases with increasing exposure. However, the exposure received from working with typical sources of ionizing radiation in the school environment is extremely small (much less than accumulated exposure from normal background). The associated risks are much much lower than the health risks of everyday activities, such as driving a car, swimming, or cycling.
At extreme ionizing radiation exposure levels, the resulting damage can exceed the body's natural repair capabilities. This can cause directly observable effects (e.g., skin burns, cataracts, or even death). However, this requires exceptionally high exposure levels which are simply unattainable from the types of ionizing radiation sources available in the school environment. Use of such sources is heavily regulated and they are only available in specialized applications (e.g., cancer treatment, nuclear power production, and industrial processing).
Sources and Types of Ionizing Radiation
The following are all common forms of ionizing radiation which may be encountered in the laboratory.
Emissions from radioactive
materials include
alpha particles
beta particles
gamma rays
Cathode Rays A cathode ray is a stream of electrons accelerated from a cathode or negatively charged electrode. If accelerated across sufficiently high voltages, the high speed stream of electrons is capable of ionization. Cathode rays are normally produced in a vacuum inside sealed glass tubes (called cathode ray tubes or CRTs).
In practice, their penetrating power is low and in most instances they are completely absorbed by the glass walls of the tube. However, at very high voltages and/or for very thin walled tubes, cathode ray leakage is possible. It is prudent practice to keep operating voltages on demonstration type tubes below 5 kV. Once cathode rays have been absorbed and given up their energy, they are simply ordinary electrons.
X rays X rays may be produced as secondary radiation from CRTs or other vacuum or discharge tubes operating at voltages above a few kV. X ray production occurs as a by-product when the accelerated electrons are absorbed. In general, X rays penetrate much more effectively than cathode rays; but in practice, their penetrating power is still sufficiently low that in most instances they are completely absorbed by the glass walls of the tube. However, as with cathode rays, at very high voltages and/or for very thin walled tubes, X-ray leakage is possible. When absorbed, the energy in an X ray is converted into other forms of energy (e.g., heat) and the X ray ceases to exist.
Radioactive Materials When dealing with ionizing radiation, it is important to remember that the terms radioactive and radiation are not interchangeable. The term radioactive specifically refers to any material which spontaneously undergoes a nuclear transition, with the concomitant emission of ionizing radiation. The radiation emitted may be in the form of a high energy particle (e.g., alpha or beta), a photon of electromagnetic energy (gamma rays), or both.
Alpha particles consist of two neutrons and two protons bound together. They are emitted at very high energies from the nuclei of some types of radioactive atoms. Because of their large size, they are easily absorbed by materials as thin as a sheet of ordinary paper. Once they have been absorbed and given up their energy, they are identical to an ordinary helium atom.
Beta particles are high speed electrons ejected from the nuclei of radioactive atoms. Physically they are comparable to cathode rays but they usually have much higher energies than the cathode rays produced in a typical CRT. Once they have been absorbed and have given up their energy, they are identical to an ordinary electron.
Gamma rays have physical properties which are identical to X rays. Gamma rays usually have much higher energies than any X rays produced by demonstration-type apparatus. As with X rays, when the absorbed energy in a gamma ray is converted into other forms of energy (e.g., heat), the photon ceases to exist.
A wide range of low activity radioactive sources are commercially available. Sealed sources usually consist of metal or plastic discs or rods containing radioactive material. Unsealed sources may be in the form of liquids or powders. Except for extremely low activity sources, an Atomic Energy Control Board (AECB) license is required before facilities can obtain radioactive sources.
Ionizing Radiation Protection Procedures
Protection of staff and students from exposure to radiation requires careful planning of experimental set ups and procedures and the maintenance of all radiation sources in good order. All potentially hazardous equipment and materials must be available for use only under the direct supervision of a teacher who is familiar with safe procedures. The onus is on the teacher to be aware of potential dangers and to convey this information to the students. The teacher must instruct students in proper operating and handling procedures and must insist that they be followed.
The two distinct types of ionizing radiation hazard are external exposure and personal contamination. Exposure refers to exposing the body to a radiation field. Ionizing radiation causes ionization within body tissue as the radiation emits energy. It is this transfer of energy via the process of ionization which results in tissue damage. Radiation exposure does not make you radioactive. Once the particle or photon has transferred its energy and been absorbed, it no longer presents a hazard. When you switch off an X-ray tube or CRT, electron acceleration and secondary X-ray production ceases immediately, there is no residual radiation, and no radioactive material is produced.
The principle methods of minimizing radiation exposure are time, distance, and shielding. Exposure is directly proportional to time: the longer the exposure the greater the potential damage and risk. Therefore, minimizing exposure time minimizes damage and risk. Exposure is inversely proportional to the square of the distance from the radiation source (i.e., inverse square law). This means that doubling the distance from the source reduces exposure to 1/4, tripling the distance reduces exposure to 1/9, and so on. Therefore, maximizing the distance from the source is an effective method of minimizing exposure. Finally, shielding simply means blocking the radiation with a suitable material. Alpha particles can be completely absorbed by very thin light materials, such as a sheet of paper. Beta particles and cathode rays are more penetrating, and may require a sheet of foil or a glass or plastic case for shielding. X rays and gamma rays are generally much more penetrating, and may require one mm to a few cm of lead for shielding.
Contamination refers to contaminating the body with radioactive material. When working with unsealed radioactive sources, it is important to make sure that the material is not spilled on the skin, ingested, or inhaled. Tissues contaminated with radioactive material are constantly being exposed to the radiation produced by that material. Once again, it is the ionization caused by this radiation which is the principle mechanism of tissue damage.
In addition to using the principles of time, distance, and shielding to minimize radiation exposure when working with unsealed radioactive sources, special precautions should be taken to minimize the potential for contamination. These precautions are similar to those for handling laboratory chemicals (i.e., wearing rubber gloves, lab coats, goggles and masks, using proper utensils to handle material, using established clean-up procedures, and eye and skin wash stations).
Finally, it is recommended that suitable radiation monitoring equipment (e.g., a pancake style Geiger-Muller detector) be available for use when working with any source of ionizing radiation. This equipment must be maintained in good working order. Training must be provided for persons using radiation monitoring equipment.
How to Cope with Spills of Radioactive Compounds
The immediate washing of contaminated areas with water and soap is the preferred method for removing loose contamination, subject to certain elementary precautions including
Washing could be repeated if necessary (as indicated by monitoring) providing there is no indication of the skin getting damaged. Attempts to remove contamination which resists mild procedures should only be made under medical supervision.
Caution: Use of organic solvents and/or acid or alkaline solutions must be avoided.
Storage of Radioactive Material
Storage of radioactive material should conform to
Transport and Disposal of Radioactive Material
Transport or shipping of any radioactive material outside of the laboratory for any reason is subject to the AECB's Transport Packaging of Radioactive Materials Regulations and to federal and provincial transport of dangerous goods regulations.
Radioactive material must be returned to the supplier for disposal or to the Waste Management Operations at Chalk River Laboratories, Atomic Energy of Canada Limited, Chalk River ON, K0J 1J0 (prior arrangements must be made. Telephone: 613-584-3311, ext. 3650, fax 613-584-1438). Any other waste disposal method requires specific approval by the Atomic Energy Control Board.
Accidents and Unusual Operating Conditions
Procedures include
Biological Effects of Non-ionizing Radiation
Human tissue interacts with this type of radiation in fundamentally different ways than it does with ionizing radiation. As its name implies, non-ionizing radiation does not ionize atoms or molecules, but can increase their kinetic energy, or "vibrate" them (i.e., transferring the radiation energy to tissues through heat production). Heat production is the main effect of exposure to infra-red, microwave, and radio frequency radiation.
At sufficiently high radiation intensities, normal physiological processes can be disrupted and, accordingly, limits on the strength of the electric and magnetic fields, or the "power density" in some cases, are specified for human exposure in publications such as Health Canada's Safety Code 6 (Limits of Exposure to Radio Frequency Fields at Frequencies from 10 kHz - 300 GHz). It is not generally necessary to measure actual field strengths in the school laboratory for comparison with the specified limits when safe laboratory practices are followed (e.g., using low intensity radiation sources and minimizing exposure), .
The tissue-penetrating ability of non-ionizing radiation is related to its wavelength. Short wavelength ultraviolet rays are absorbed by the skin and the cornea and conjunctiva of the eye, causing a burn-like reaction when the radiation intensity or combination of intensity and exposure time is sufficiently high. Sunburn and the painful "welder's flash" burn of the eye are familiar effects of ultraviolet radiation exposure. In contrast to this acute type of radiation-induced injury, prolonged or chronic exposure to ultraviolet radiation may result in more serious effects such as premature skin aging or skin cancer.
Sources and Types of Non-ionizing Radiation
Types that may be found in school laboratories include:
Visible light (including spectroscopic sources such as mercury, hydrogen, iodine, and sodium vapour discharge tubes)
Ultraviolet Lamps and Electric Arcs Ultraviolet lamps, such as those used to detect the presence of certain compounds by their fluorescence, must be used in such a way that the source can never be viewed directly. Electric arcs produce very high intensities of ultraviolet radiation and must never be used as an open source. If an arc is used to provide intense visible light, it must be enclosed except for an exit pupil where a filter can be used to absorb ultraviolet light from the desired visible light beam.
Lasers The visible beam of light from a laser is focussed by the lens of the eye and can cause severe retinal damage with very brief exposure when the laser is of sufficient power. For this reason, the Canadian Radiation Emitting Devices Regulation specifies that demonstration lasers for educational institutions be limited to 1 milliwatt beam power and be within the wavelength range from 400 to 780 nanometres (visible).
For lasers meeting these criteria, the normal blink response time (0.25 seconds) of the eye is sufficient to prevent retinal damage. In addition, lasers must be used under the close direction of a teacher, usually in a well-lit room so that the pupils of the eye are small. Lasers should be positioned in such a way that the beam cannot enter a person's eye, either directly or by specular (mirror-like) reflection. It should also be noted that the direct or reflected viewing of any intense visible light source -- such as electric arcs, burning magnesium ribbon, the Sun, and collimated or focussed beams from ordinary tungsten lights -- can cause retinal damage.
Microwave Generators The microwave radiation generators used in school laboratories for demonstration purposes produce low intensity microwave radiation. Exposure to microwave radiation may produce biological effects (such as tissue heating). Care should be taken to minimize the exposure of students and staff through measures such as preventing access to the beam by shielding its path and restricting admittance to the area through which the beam passes.
New microwave ovens sold in Canada must not leak radiation above levels specified in the Canadian Radiation Emitting Devices Regulations. Ovens used in cafeterias, and home economics and science laboratories may present a hazard from microwave radiation leakage if the door or door seals have been damaged through use (e.g., closing the door accidentally on ovenware items prevents the original tight door seal). If damage to an oven has occurred or is suspected, leakage radiation levels should be measured.
Infra-red and Radio Frequency Infra-red and radio frequency radiation sources used in schools are usually of low intensity and used only occasionally. If these practices continue, infra-red and radio frequency radiation do not appear to present significant hazards. Any change in these practices should be accompanied by appropriate precautions to reduce the exposure of students and the teacher to the radiation.
Non-ionizing Radiation Protection Procedures
Protection of staff and students from exposure to non-ionizing radiation requires careful planning of experimental set-ups and procedures and the maintenance of all radiation sources in good order. All potentially hazardous equipment and materials must be available for use only under the direct supervision of a teacher who is familiar with safe procedures. The onus is on the teacher to be aware of potential dangers and to convey this information to the students. The teacher must instruct students in proper operating and handling procedures and must insist that they be followed.
The basic objective of radiation protection is to minimize the radiation exposure of persons who are required to handle radiation sources. Measures that can be used with non-ionizing radiation include
Accidents and Unusual Operating Conditions
Procedures include